Description
The GloResponse™ NFAT-RE- luc2P HEK293 Cell Line is a clonal derivative of Human Embryonic Kidney 293 (HEK293) cells. These cells contain a luciferase gene ( luc2P ) under the control of a minimal TATA promoter with multiple Nuclear Factor of Activated T-cell response elements (NFAT-REs). NFAT-REs are the DNA-binding sequences for the NFAT family of transcription factors, which are responsible for regulating a variety of biological functions including cell proliferation, cytokine production, and cardiovascular development. The GloResponse™ NFAT-RE- luc2P HEK293 Cell Line is designed for rapid and convenient analysis of any cellular response that results in modulation of NFAT activities.
GPCRs regulate a wide-range of biological functions and are one of the most important target classes for drug discovery. GPCR signaling pathways can be categorized into three classes based on the G protein α-subunit involved: Gs, Gi/o and Gq. The GloResponse™ NFAT-RE- luc2P HEK293 Cell Line can be used for Gq-coupled GPCRs, which signal through calcium ion release and activate the Nuclear Factor of Activated T-Cells response element (NFAT-RE).
The GloResponse™ Cell Lines incorporate the improvements developed for the pGL4 family of reporter vectors for enhanced performance. The destabilized luc2P luciferase reporter is used for improved responsiveness to transcriptional dynamics. The luc2P gene is codon optimized for enhanced expression in mammalian cells, and the pGL4 plasmid backbone was engineered to reduce background reporter expression. The result is a cell line with very high induction levels when the pathway of interest is activated.
GloResponse™ NFAT-RE- luc2P HEK293 cells response to PMA titration. A total of 10,000 GloResponse™ NFAT-RE- luc2P HEK293 cells per well were dispensed into each well of a 384-well plate, and threefold serial dilutions of PMA were added to induce reporter gene expression. After 16 hours of induction in a tissue culture incubator, luciferase activity was quantified using the Dual-Glo® Luciferase Assay System Reagent on the Berthold® LB 96 V Luminometer. n = 8 for each data point.
