Promega, G2930, Griess Reagent System

Name: Griess Reagent System
Brand: Promega
SKU: G2930

Catalog Number: G2930

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TagsCell Health Assays Oxidative Stress Assays

Description

The Griess Reagent System measures nitrite (NO 2 – ), one of two primary stable and nonvolatile breakdown products of nitric oxide (NO). Nitric oxide is an important physiological messenger and effector molecule in many biological systems, including immunological, neuronal and cardiovascular tissues. This assay relies on a diazotization reaction that was originally described by Griess in 1879. Through the years, many modifications to the original reaction have been described.
The Griess Reagent System is based on a chemical reaction that uses sulfanilamide and N-1-naphthylethylenediamine dihydrochloride (NED) under acidic (phosphoric acid) conditions. This system detects NO 2 – in a variety of biological and experimental liquid matrices such as plasma, serum, urine and tissue culture medium. The nitrite sensitivity is dependent on the matrix. The limit of detection is 2.5μM (125pmol) nitrite (in ultrapure, deionized, distilled water) using the protocol described in Technical Bulletin #TB229.
Original reference: Griess, P. (1879) Chem. Ber. 12 , 426–8.

Specifications

General

Catalog Number	G2930

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