Promega, G6080, CellTiter-Fluor™ Cell Viability Assay

Multiplexing with the CellTiter-Fluor™ Assay
The CellTiter-Fluor™ Assay also can be used in a single-well, sequential, multiplex format with other downstream assay chemistries to normalize data by cell number. Data from the assay can serve as an internal control and allow identification of errors resulting from cell clumping or compound cytotoxicity. In the example data on the right, ionomycin and PMA work cooperatively to stimulate NFAT-dependent gene expression; however, a higher concentration of ionomycin results in decreased expression. By simultaneously monitoring viability with the CellTiter-Fluor™ Assay, it is shown that the decrease in expression is due to a decrease in viable cells.
Luciferase reporter expression (ONE-Glo™ Assay) and cell viability (CellTiter-Fluor™ Assay) at increasing ionomycin concentration.
The CellTiter-Fluor™ Reagent was added to wells and viability measured after incubation for 30 minutes at 37°C. Caspase-Glo® 3/7 Reagent was added and luminescence measured after a 30-minute incubation (10,000 cells/well).