Promega, G6270, HaloTag® Protein Purification System

Enhanced Expression and Solubility of Purified Protein with Novel Tag
HaloTag® Protein Purification System is designed to purify proteins fused to the HaloTag® novel protein tag that enhances the expression and solubility of recombinant proteins. HaloTag® Technology enables the covalent, efficient and specific capture of a protein of interest onto HaloLink™ Resin, thus overcoming the equilibrium-based limitations associated with affinity tags (i.e., poor capture of proteins expressed at low levels and protein loss during washing of the purification resin).
The HaloTag® technology offers a quick and convenient way to test protein expression of HaloTag® fusion proteins as well as to monitor the efficiency of immobilization to HaloLink™ Resin by labeling with fluorescent HaloTag® TMR Ligand followed by SDS-PAGE analysis. Guidelines can be found in the HaloLink ™ Resin Technical Manual #TM250 .
Use the HaloTag® Protein Purification System for:
Purification of poorly expressed fusion proteins
Improved yield, purity and specific activity of soluble, functional proteins compared to His-Tag, GST and MBP affinity tags
Enhance target protein expression in prokaryotic, mammalian and cell-free systems
Recover tag-free target protein using TEV Protease cleavage
In-gel detection and quantification of protein expression levels with fluorescent HaloTag® Ligands
Schematic of protein purification with HaloTag® fusion tagging.
Use of HaloTag® protein improved yields compared to yields from other tags.