Promega, G8282, HaloTag® Ligands for Protein Purification

Optimized Protein Purification and Capture with HaloTag® Ligands
The Promega HaloTag® Ligand portfolio enables a range of functionalities, including affinity labeling for protein purification. These ligands form highly specific and nearly irreversible covalent bonds with HaloTag® fusion proteins under physiological conditions, ensuring stable and efficient labeling with minimal background interference. HaloTag® Ligands enable you to study any compound or surface with a compatible chemical group. With a full suite of HaloTag® Ligand Building Blocks , the possible applications are endless.
HaloTag® Biotin Ligands enable efficient biotin labeling for protein capture and analysis. The HaloTag® Biotin Ligand is cell-permeant, allowing intracellular labeling while unbound ligand can be washed away after incubation.
For enhanced pull-down performance, the HaloTag® PEG-Biotin Ligand offers superior interaction with streptavidin due to its extended and flexible spacer. While it requires cell lysis prior to labeling due to limited membrane permeability, this extended linker can help preserve the activity of HaloTag® fusion proteins during immobilization or derivatization.
Interested in direct capture of HaloTag? Learn about our HaloLink™ Resin .