Promega, G8451, pFN28A and pFN28K HaloTag® CMV-neo Flexi® Vectors

Constitutive Protein Expression in Mammalian Cells
The pFN28A and pFN28K HaloTag® CMV-neo Flexi Vectors append the HaloTag® protein to the aminoterminus of the protein fusion partner. The vectors provide constitutive high-level protein expression in mammalian cells using the CMV immediate-early enhancer/promoter. The vectors can be used for both transient and stable gene expression. The stable expression is mediated by co-expression of the neomycin phosphotransferase gene, which confers resistance to the Antibiotic G-418 Sulfate (Cat.# V7983) under the control of an SV40 promoter, allowing selection of stable transfectants.
Note: Flexi® Vectors contain the lethal barnase gene to reduce background colonies without inserts during the subcloning procedure. Using the Flexi® Vector Cloning System replaces the barnase gene with your insert. These vectors, as purchased, cannot be cultured in normal laboratory strains of E. coli without an insert.
Clone ORF and conveniently transfer these inserts to a variety of vectors designed for multiple applications.
GenBank® Accession Numbers and Sequence Information
pFN28A HaloTag® CMV-neo GenBank® Accession Number JN129497 ; pFN28A HaloTag® CMV-neo Vector sequence text file . pFN28K HaloTag® CMV-neo GenBank® Accession Number JN129498 ; pFN28K HaloTag® CMV-neo Vector sequence text file .