Promega, G9061, NAD(P)H-Glo™ Detection System

Name: NAD(P)H-Glo™ Detection System
Brand: Promega
SKU: G9061

Monitor the Concentration of Reduced Forms of NADH and NADPH, Highly sensitive and rapid bioluminescent assay, Easily adapted for high-throughput format, Simple add-mix-measure protocol, Configuration: 10ml

Catalog Number: G9061

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Category

IHC Detection Systems

TagsMetabolic Activity Assays Nucleotide and Co-Factor Detection Assays

Description

The NAD(P)H-Glo™ Detection System is a bioluminescent, homogeneous, single-reagent-addition assay that quantitatively monitors the concentration of the reduced forms of NADH and NADPH levels in enzymatic reactions. The oxidized forms, NAD+ and NADP+, are not detected and do not interfere with quantitation.
The assay is rapid, requiring only a 40- to 60-minute incubation, has a broad linear range (25nM-50µM) and high signal-to background ratio (~400). Low nanomolar sensitivity, 25nM, allows direct in-well detection. The assay is well suited to measuring NAD(P)H production or consumption in high-throughput formats.
Assay Chemistry
In the presence of NAD(P)H, a reductase reduces the proluciferin reductase substrate to form luciferin. Luciferin is quantified using Ultra-Glo™ Recombinant Luciferase, which is a component of the Luciferin Detection Reagent, and the light signal produced is proportional to the amount of NAD(P)H in the sample.

Specifications

{"type": "table", "rows": [{"name": "Reductase", "conditions": {"Part #": "G884A", "Size": "1 × 55μl", "Concentration": "6mg/ml"}}, {"name": "Reductase Substrate", "conditions": {"Part #": "G885A", "Size": "1 × 55μl", "Concentration": ""}}, {"name": "Luciferin Detection Reagent", "conditions": {"Part #": "V859A", "Size": "1 × 1 each", "Concentration": ""}}, {"name": "Reconstitution Buffer", "conditions": {"Part #": "V865A", "Size": "1 × 10ml", "Concentration": ""}}]}

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