Promega, G9071, NAD/NADH-Glo™ and NADP/NADPH-Glo™ Assays

Name: NAD/NADH-Glo™ and NADP/NADPH-Glo™ Assays
Brand: Promega
SKU: G9071

Bioluminescent Assays to Detect NAD(P)+ and NAD(P)H Levels in Cells, Homogeneous, single-reagent-addition assays, Easily adapted for high-throughput screening formats, Configuration: NAD/NADH/10ml

Catalog Number: G9071

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Category

Endotoxin Detection Reagents

Pack Size1 vial

TagsMetabolic Activity Assays Nucleotide and Co-Factor Detection Assays

Description

NAD/NADH-Glo™ Assay and NADP/NADPH-Glo™ Assays
The NAD/NADH-Glo and NADP/NADPH-Glo Assays are bioluminescent, homogeneous, single-reagent-addition methods to rapidly detect NAD+ and NADH and NADP+ and NADPH levels in cells and enzymatic reactions. Current methods to detect nicotinamide adenine dinucleotides lack sensitivity and reproducibility and require sample manipulation and large numbers of cells for cell-based applications. The assay is easily adapted for inhibitor screening in high-throughput formats. The sensitivity and robustness of the assay chemistry allow fewer cells to be used to detect individual nucleotides directly in multiwell plates or analysis of enzymes with low activity or with low Km values.

Specifications

{"type": "table", "rows": [{"name": "Reductase", "conditions": {"Part #": "G884A", "Size": "1 × 55μl", "Concentration": "6mg/ml"}}, {"name": "Reductase Substrate", "conditions": {"Part #": "G885A", "Size": "1 × 55μl", "Concentration": ""}}, {"name": "NAD Cycling Enzyme (lyophilized)", "conditions": {"Part #": "G887A", "Size": "1 × 1 vial", "Concentration": ""}}, {"name": "NAD Cycling Substrate", "conditions": {"Part #": "G888A", "Size": "1 × 1.25ml", "Concentration": ""}}, {"name": "Luciferin Detection Reagent", "conditions": {"Part #": "V859A", "Size": "1 × 1 each", "Concentration": ""}}, {"name": "Reconstitution Buffer", "conditions": {"Part #": "V865A", "Size": "1 × 10ml", "Concentration": ""}}]}

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