Promega, G9712, RealTime-Glo™ MT Cell Viability Assay

Save Time, Cell Culture and Reagent Costs
One RealTime-Glo™ Assay Plate Yields the Same Data as Many Endpoint Assay Plates
Five hundred A549 cells were plated in medium containing the RealTime-Glo™ MT Reagents in 384-well plates and various concentrations of bortezomib. The luminescence signal was determined from each well every hour for 72 hours in a Tecan M200 plate reader with gas control module (37°C/5% CO 2 ).
Data from specific time points plotted to calculate EC 50 at these time points.
Rapidly Detect Changes in Cell Viability
The RealTime-Glo™ MT Cell Viability Assay generates a luminescent signal that is dependent upon continuous reduction of the MT cell viability substrate by viable cells and rapid turnover by NanoLuc® Luciferase. Conventional assays based on reducing potential (alamarBlue, MTT) are accumulation-based and do not allow real-time detection of cell death in the same well. In this experiment, A549 cells were treated with 1% Triton® X-100. Viability assay reagents were applied and incubated for the indicated times post-treatment.