Promega, G9790, ADCC Reporter Bioassay, F Variant

Name: ADCC Reporter Bioassay, F Variant
Brand: Promega
SKU: G9790

MOA-Based Bioassay to Measure FcγRIIIa (F158) Function, No cell culture required, Quantitate impact of F158 polymorphism on Fc effector function, Supplied in convenient thaw-and-use format; also available in cell propagation model (CPM) format, Configuration: Core Kit/1X each component

Catalog Number: G9790

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Category

Cell-Free Protein Expression Kits

Pack Size1 vial

TagsBioassays Fc Effector Activity Bioassays

Description

The Pathway
The human FcyRIIIa gene displays a polymorphism in the position coding for amino acid residue 158. This translates to a higher affinity FcyRIIIa variant having a valine at amino acid 158 (V158 and a lower affinity FcyRIIIa variant carrying phenylalanine F158). Killing of target cells is an endpoint of this pathway activation and is used in classic ADCC bioassays, which use donor peripheral blood mononuclear cells (PBMC) or the natural killer (NK) cell subpopulation as effector cells isolated from blood donors with FcyRIIIa VV, VF or FF alleles.
These primary cells can be highly variable in response, are tedious to prepare and can result in high background readings. It is highly challenging to quantitatively evaluate the impact of FcyRIIIa polymorphism by classic in vitro ADCC bioassays. The ADCC Reporter Bioassay, F Variant, overcomes many of these pain points.

Specifications

{"type": "table", "rows": [{"name": "ADCC Bioassay Effector Cells, F Variant", "conditions": {"Part #": "G979A", "Size": "1 × 1 vial"}}, {"name": "Low IgG Serum", "conditions": {"Part #": "G711A", "Size": "1 × 4ml"}}, {"name": "RPMI 1640 Medium", "conditions": {"Part #": "G708A", "Size": "1 × 36ml"}}, {"name": "Bio-Glo™ Luciferase Assay Buffer", "conditions": {"Part #": "G719A", "Size": "1 × 10ml"}}, {"name": "Bio-Glo™ Luciferase Assay Substrate", "conditions": {"Part #": "G720A", "Size": "1 × 1 vial"}}]}

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