Promega, G9901, FcγRIIa-H ADCP Bioassay

Name: FcγRIIa-H ADCP Bioassay
Brand: Promega
SKU: G9901

Measure Potency and Stability of Biologics that Bind and Activate ADCP through FcγRIIa-H, Complete format contains everything you need to get started, Use Core format with customer-defined Ab and target cells, Also available in cell propagation model (CPM) format, Configuration: Complete Kit/1 each

Catalog Number: G9901

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Category

Cell-Free Protein Expression Kits

Pack Size1 vial

TagsBioassays Fc Effector Activity Bioassays

Description

Measure Potency and Stability of Biologics that Bind and Activate FcyRIIa-H
Antibody-dependent cell-mediated phagocytosis (ADCP) is an important mechanism of action (MOA) of therapeutic antibodies. In vivo, ADCP can be mediated by monocytes, macrophages, neutrophils and dendritic cells via FcγRIIa, FcγRI and FcγRIIIa. While all three receptors can participate in ADCP, FcγRIIa is believed to be the predominant Fcγ receptor involved in this process.
The FcγRIIa-H ADCP Reporter Bioassay is a biologically relevant MOA-based assay that can be used to measure the potency and stability of antibodies and other biologics that specifically bind and activate FcγRIIa. The assay consists of Jurkat cells stably expressing human FcγRIIa-H (the high-affinity H131 variant) and NFAT-induced luciferase.
The bioassay workflow is simple and robust, compatible with 96-well and 384-well plate formats, and unlike traditional primary cell-based assays, amenable for use in quality-controlled drug development settings.

Specifications

{"type": "table", "rows": [{"name": "FcγRIIa-H Effector Cells", "conditions": {"Part #": "G988A", "Size": "1 × 0.62ml"}}, {"name": "Target Cells (Raji)", "conditions": {"Part #": "G960A", "Size": "1 × 0.55ml"}}, {"name": "ADCP Control Ab, Anti-CD20", "conditions": {"Part #": "G989A", "Size": "1 × 2.5μg"}}, {"name": "Low IgG Serum", "conditions": {"Part #": "G711A", "Size": "1 × 4ml"}}, {"name": "RPMI 1640 Medium", "conditions": {"Part #": "G708A", "Size": "1 × 36ml"}}, {"name": "Bio-Glo™ Luciferase Assay Buffer", "conditions": {"Part #": "G719A", "Size": "1 × 10ml"}}, {"name": "Bio-Glo™ Luciferase Assay Substrate", "conditions": {"Part #": "G720A", "Size": "1 × 1 vial"}}]}

Ordering

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