Promega, G9995, FcγRIIa-H ADCP Bioassay

Name: FcγRIIa-H ADCP Bioassay
Brand: Promega
SKU: G9995

Measure Potency and Stability of Biologics that Bind and Activate ADCP through FcγRIIa-H, Complete format contains everything you need to get started, Use Core format with customer-defined Ab and target cells, Also available in cell propagation model (CPM) format, Configuration: Core Kit/5X each component

Catalog Number: G9995

Pricing

Send an RFQ.

View RFQ List

Category

Cell-Free Protein Expression Kits

Pack Size1 vial

TagsBioassays Fc Effector Activity Bioassays

Description

Measure Potency and Stability of Biologics that Bind and Activate FcyRIIa-H
Antibody-dependent cell-mediated phagocytosis (ADCP) is an important mechanism of action (MOA) of therapeutic antibodies. In vivo, ADCP can be mediated by monocytes, macrophages, neutrophils and dendritic cells via FcγRIIa, FcγRI and FcγRIIIa. While all three receptors can participate in ADCP, FcγRIIa is believed to be the predominant Fcγ receptor involved in this process.
The FcγRIIa-H ADCP Reporter Bioassay is a biologically relevant MOA-based assay that can be used to measure the potency and stability of antibodies and other biologics that specifically bind and activate FcγRIIa. The assay consists of Jurkat cells stably expressing human FcγRIIa-H (the high-affinity H131 variant) and NFAT-induced luciferase.
The bioassay workflow is simple and robust, compatible with 96-well and 384-well plate formats, and unlike traditional primary cell-based assays, amenable for use in quality-controlled drug development settings.

Specifications

{"type": "table", "rows": [{"name": "FcγRIIa-H Effector Cells", "conditions": {"Part #": "G988A", "Size": "5 × 0.62ml"}}, {"name": "Low IgG Serum", "conditions": {"Part #": "G711A", "Size": "5 × 4ml"}}, {"name": "RPMI 1640 Medium", "conditions": {"Part #": "G708A", "Size": "5 × 36ml"}}, {"name": "Bio-Glo™ Luciferase Assay Buffer", "conditions": {"Part #": "G719A", "Size": "5 × 10ml"}}, {"name": "Bio-Glo™ Luciferase Assay Substrate", "conditions": {"Part #": "G720A", "Size": "5 × 1 vial"}}]}

Ordering

Notice

Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:

[email protected]

Please send inquiries directly to us in the “To” field. Emails sent via BCC, mass mailing, or with unrelated recipients may be automatically filtered and will not be processed.