Promega, GA1050, Autophagy LC3 HiBiT Reporter Assay System

Name: Autophagy LC3 HiBiT Reporter Assay System
Brand: Promega
SKU: GA1050

Catalog Number: GA1050

Pricing

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Category

Western Blot Detection Reagents

TagsCell Health Assays Autophagy Detection

Description

How to Measure Autophagy Using the LC3 Assay
The Autophagy LC3 HiBiT Reporter consists of a HiBiT tag fused to the human LC3 protein, connected by a HaloTag® spacer. When autophagy is induced, LC3 reporter proteins are captured in autophagosomes and degraded. With addition of the Nano-Glo® HiBiT Lytic Reagent containing LgBiT protein and substrate, LgBiT interacts with the HiBiT tag to reconstitute the bright, luminescent NanoBiT® enzyme. The luminescent signal is directly proportional to the level of HiBiT-tagged LC3 reporter over seven orders of magnitude. Additional detection modules are HiBiT blotting and i maging with HaloTag® ligands.

Specifications

General

Catalog Number	GA1050

Ordering

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