Promega, J2232, FcγRIIb CHO-K1 Cells

Name: FcγRIIb CHO-K1 Cells
Brand: Promega
SKU: J2232

Measure Potency and Stability of Agonist Antibodies that are FcγRIIb Crosslinking-Dependent, Used with co-stimulatory bioassays to crosslink antibodies that stimulate T cell activation, Measure FcγRIIb crosslinking dependence of agonist antibodies, Mechanism-of-action-based assay, Thaw-and-use format to achieve consistency; also available in cell propagation model (CPM) format, Configuration: Cell Propagation Model/2 × 1.0ml

Catalog Number: J2232

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Category

Cell Cycle Reagents

TagsBioassays Immune Checkpoint Bioassays

Description

The Assay Principle
Representation of co-stimulatory bioassays (CD40, OX40, GITR or 4-1BB) with FcγRIIb-independent and FcγRIIb-dependent antibodies. Panel A. Assay with FcγRIIb-independent antibody. The bioassay consists of one engineered cell line: CD40, OX40, GITR or 4-1BB Effector Cells. In the absence of agonist antibody or ligand, the CD40, OX40, GITR or 4-1BB receptor is not activated and luminescence signal is low. The addition of agonist antibody or ligand induces the CD40, OX40, GITR or 4-1BB pathway-activated luminescence, which can be detected in a dose-dependent manner by addition of Bio-Glo™ Reagent and quantitation with a luminometer. Panel B. Assay with FcγRIIb-dependent antibody. The bioassay consists of two engineered cell lines: CD40, OX40, GITR or 4-1BB Effector Cells and FcγRIIb CHO-K1 Cells. In the presence of FcγRIIb CHO-K1 Cells, the anti-CD40, -OX40, -GITR or 4-1BB antibody is cross-linked, thereby inducing CD40, OX40, GITR or 4-1BB pathway-activated luminescence, which can be detected in a dose-dependent manner by addition of Bio-Glo™ Reagent and quantitation with a luminometer.

Specifications

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Ordering

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