Promega, J3150, Glycerol-Glo™ Assay

Name: Glycerol-Glo™ Assay
Brand: Promega
SKU: J3150

Simple, Sensitive Glycerol Detection Assay for a Variety of Biological Samples, Works with lysed cells, tissues, medium and serum samples, Perform directly in-well, Linear glycerol detection up to 80µM, Configuration: 5ml

Catalog Number: J3150

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Category

Lipid Supplements

TagsMetabolic Activity Assays Lipid Metabolism

Description

How the Assay Works
The Glycerol-Glo™ Assay measures glycerol in a coupled reaction scheme that links the production of NADH to the activation of a proluciferin that produces light with luciferase. Glycerol kinase and glycerol-3-phosphate dehydrogenase are used to generate NADH.
In the presence of NADH, Reductase enzymatically reduces a proluciferin Reductase Substrate to luciferin. Luciferin is detected in a luciferase reaction using Ultra-Glo™ Luciferase and ATP and the amount of light produced is proportional to the amount of glycerol in the sample.
Simple, Flexible Sample Preparation
Glycerol Release from Adipocytes
The Glycerol-Glo™ Assay can be used with cultured cells, including 3D cell culture models, homogenized tissue samples, cell culture medium samples and serum or plasma samples.
No organic extraction is required. The Glycerol Lysis Reagent effectively extracts glycerol from your sample in a single reagent addition without extreme heat, centrifugation steps or multi-step organic extraction protocols.

Specifications

{"type": "table", "rows": [{"name": "Reductase Substrate", "conditions": {"Part #": "G885A", "Size": "1 × 55μl", "Concentration": ""}}, {"name": "Glycerol Lysis Solution", "conditions": {"Part #": "J315A", "Size": "1 × 10ml", "Concentration": ""}}, {"name": "Kinetic Enhancer", "conditions": {"Part #": "J316A", "Size": "1 × 50μl", "Concentration": ""}}, {"name": "Glycerol Detection Solution", "conditions": {"Part #": "J317A", "Size": "1 × 5ml", "Concentration": ""}}, {"name": "Glycerol Standard", "conditions": {"Part #": "J324A", "Size": "1 × 500μl", "Concentration": "20mM"}}]}

Ordering

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