Promega, J4051, Pyruvate-Glo™ Assay

Name: Pyruvate-Glo™ Assay
Brand: Promega
SKU: J4051

Easy, Sensitive Pyruvate Assay Kit, Compatible with cells, media and serum samples, Streamlined in-well sample preparation protocol, Scalable to 384-well plates for HTS applications, Configuration: 5ml

Catalog Number: J4051

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Category

Coliform Detection Kits

TagsMetabolic Activity Assays Metabolite Detection Assays

Description

Measure the Effect of Metabolic Inhibitors on Cellular Pyruvate Levels
The Pyruvate-Glo™ Assay can be used to monitor both intracellular and extracellular pyruvate in cell culture, including quickly assessing the metabolic effects of drug treatment.
Monitor metabolic effects of drug treatment with the Pyruvate-Glo™ Assay. K562 cells in suspension were incubated with DMSO (negative control), 10μM GSK2837808 (lactate dehydrogenase A inhibitor), 10μM 7ACC2 (monocarboxylate transporter 1-4 [MCT1-4] inhibitor) or 10μM UK5099 (mitochondrial pyruvate carrier inhibitor) for one to two hours. Following the incubations, half the volume was analyzed using the Pyruvate-Glo™ Assay ( Panel A ), and half the volume was removed for viability analysis using CellTiter-Glo® Luminescent Cell Viability Assay ( Panel B ). As expected, pyruvate levels increased approximately twofold after a 2-hour incubation with UK5099 and 7ACC2 compared to the DMSO control. There was no decrease in viability during treatment when compared to the DMSO control. All data represent an average of three replicates.

Specifications

{"type": "table", "rows": [{"name": "H 2 O 2 Substrate, 10mM", "conditions": {"Part #": "G882A", "Size": "1 × 40μl"}}, {"name": "Signal Enhancer Solution", "conditions": {"Part #": "G883A", "Size": "1 × 100μl"}}, {"name": "H 2 O 2 Substrate Dilution Buffer", "conditions": {"Part #": "G922A", "Size": "1 × 2ml"}}, {"name": "Luciferin Detection Solution", "conditions": {"Part #": "J135A", "Size": "1 × 5ml"}}, {"name": "Neutralization Buffer", "conditions": {"Part #": "J153A", "Size": "1 × 15ml"}}, {"name": "0.6N HCl", "conditions": {"Part #": "J402A", "Size": "1 × 15ml"}}, {"name": "Pyruvate Oxidase (POX)", "conditions": {"Part #": "J416A", "Size": "1 × 130μl"}}, {"name": "Pyruvate, 10mM", "conditions": {"Part #": "J417A", "Size": "1 × 50μl"}}, {"name": "d -Cysteine, 100X", "conditions": {"Part #": "V251A", "Size": "1 × 100μl"}}]}

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