Promega, J9020, Dehydrogenase-Glo™ Detection System

Name: Dehydrogenase-Glo™ Detection System
Brand: Promega
SKU: J9020

Sensitive Detection of Dehydrogenase Enzyme Activity, Bioluminescent NAD(P)H detection toolkit that can be implemented rapidly to measure dehydrogenase activity in biological and biochemical samples, Extremely sensitive detection from just 10s of cells, Enables functional analysis of all major metabolic pathways, Configuration: 50ml

Catalog Number: J9020

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Category

IHC Detection Systems

TagsMetabolic Activity Assays Nucleotide and Co-Factor Detection Assays

Description

Measuring Dehydrogenase Activity in K562 Cells
Measuring dehydrogenase activity in K562 cells. K562 cells were diluted twofold starting at 2.5 × 10 4 cells/ml in PBS, and 50μl was transferred into a 96-well assay plate. Dehydrogenase detection reagent was prepared for each dehydrogenase, and 50μl was added to sample wells. No-substrate controls (NAD only and NADP only) were included to determine background from nonspecific enzyme activity in K562 cells. Panel A. Luminescence was measured for dehydrogenases and controls after 60 minutes at different cell densities. Panel B. Time-dependent increase in GAPDH activity was shown at different cell densities. Luminescence was read every 15 minutes for 120 minutes. Solid black lines indicate linear range for each cell number. Panel C. Specific activity for each dehydrogenase was calculated. Data represent the average of three replicates with error bars indicating one standard deviation.

Specifications

{"type": "table", "rows": [{"name": "Reductase", "conditions": {"Part #": "G884B", "Size": "1 × 275μl", "Concentration": "6mg/ml"}}, {"name": "Reductase Substrate", "conditions": {"Part #": "G885B", "Size": "1 × 275μl", "Concentration": ""}}, {"name": "Luciferin Detection Solution", "conditions": {"Part #": "J135B", "Size": "1 × 50ml", "Concentration": ""}}, {"name": "NAD", "conditions": {"Part #": "J136C", "Size": "1 × 1ml", "Concentration": ""}}, {"name": "NADP+", "conditions": {"Part #": "J154C", "Size": "1 × 500μl", "Concentration": ""}}]}

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