Promega, JA2195, OX40 Bioassay

Assay Performance Data
Accuracy and precision are critical to bioassays and the development of biologics. This assay was developed and prequalified according to ICH guidelines for accuracy and precision, giving it the performance characteristics required for many applications of a bioassay used for antibody drug discovery research and development. Accuracy: Measure of how close results are to a reference or "target" value. IntraLab Precision: Measure of repeatability from multiple assays of the same homogeneous sample under the same operating conditions over a short time (same analyst; same lab; same day). Intermediate Precision: Measure of repeatability of multiple assays of the same homogeneous sample with different analysts or equipment or on different days. Linearity: Ability of the assay to provide measured values that are proportional to the concentration of the sample.
The OX40 Bioassays Show Precision, Accuracy and Linearity.
Panel A. OX40 Effector Cells were induced with a serial titration of the following ligands: GITRL crosslinked by anti-HA Ab; 4-1BBL crosslinked by anti-His Ab; and OX40L. After a 5-hour induction, Bio-Glo™ Reagent was added, and luminescence was quantified using the GloMax® Discover System.
Panel B. OX40 Effector Cells were induced with a titration of Control Ab, Anti-GITR (Cat.# K1171), Control Ab, Anti-4-1BB (Cat.# K1161) and Control Ab, Anti-OX40 (Cat.# K1191), in the presence of FcγRIIb CHO-K1 Cells (Cat.# JA2232). After a 5-hour induction, Bio-Glo™ Reagent was added, and luminescence was quantified using the GloMax® Discover System.