Promega, JA2505, IL-6 Bioassay

Name: IL-6 Bioassay
Brand: Promega
SKU: JA2505

A Simpler, More Consistent IL-6 Bioassay, Prequalified according to ICH guidelines, 96- and 384-well plate formats, No primary cell culture required, Supplied in convenient thaw-and-use format; also available in cell propagation model (CPM) format, Configuration: 5 × Each Component

Catalog Number: JA2505

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Category

Sample Acquisition Plates

Pack Size1 vial

TagsBioassays Cytokine and Growth Factor Assays

Description

Assay Performance Data
Accuracy and precision are critical to bioassays and the development of biologics. This assay was developed and prequalified according to ICH guidelines for accuracy and precision, giving it the performance characteristics required for many applications of a bioassay used for antibody drug discovery research and development. Accuracy: Measure of how close results are to a reference or "target" value. IntraLab Precision: Measure of repeatability from multiple assays of the same homogeneous sample under the same operating conditions over a short time (same analyst; same lab; same day). Intermediate Precision: Measure of repeatability of multiple assays of the same homogeneous sample with different analysts or equipment or on different days. Linearity: Ability of the assay to provide measured values that are proportional to the concentration of the sample.
The IL-6 Bioassay Shows Precision, Accuracy and Linearity.
The IL-6 Bioassay responds to recombinant IL-6.
IL-6 Bioassay Cells were grown and prepared as described in the protocol, and incubated with serial dilutions of recombinant IL-6. After a 4-, 5-, 6- or 24-hour incubation, Bio-Glo™ Reagent was added, and luminescence was quantified using the GloMax® Discover System.
The IL-6 Bioassay indicates stability.
Tocilizumab (anti-IL-6R) was treated with various concentrations of hydrogen peroxide (0–0.05%) for 18 hours at 26°C prior to use in the IL-6 Bioassay. Bio-Glo™ Reagent was added, and luminescence was quantified using the GloMax® Discover System.

Specifications

{"type": "table", "rows": [{"name": "IL-6 Bioassay Cells", "conditions": {"Part #": "J302A", "Size": "5 × 0.65ml"}}, {"name": "Fetal Bovine Serum", "conditions": {"Part #": "J121A", "Size": "5 × 4ml"}}, {"name": "RPMI 1640 Medium", "conditions": {"Part #": "G708A", "Size": "5 × 36ml"}}, {"name": "Bio-Glo™ Luciferase Assay Buffer", "conditions": {"Part #": "G719A", "Size": "5 × 10ml"}}, {"name": "Bio-Glo™ Luciferase Assay Substrate", "conditions": {"Part #": "G720A", "Size": "5 × 1 vial"}}]}

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