Promega, JA5101, IL-2Rβγ Bioassay

Assay Performance Data
Accuracy and Precision are critical to bioassays and the development of biologics. The IL-2Rβγ Bioassay is both accurate and precise. Accuracy is a measure of how close results are to a target or reference value. Precision is a measure of repeatability. This assay was developed and prequalified according to ICH guidelines for accuracy and precision, giving it the performance characteristics required for many applications of a bioassay used for antibody drug discovery research and development.
Accuracy: Measure of ability to replicate a reference or "target" value.
IntraLab Precision: Measure of repeatability from multiple assays of the same homogeneous sample under the same operating conditions over a short time (same analyst; same lab; same day).
Intermediate Precision: Measure of repeatability of multiple assays of the same homogeneous sample with different analysts or equipment or on different days.
Linearity: Ability of your assay to provide measured values that are proportional to the concentration of the sample.
The IL-2Rβγ Bioassay Shows Precision, Accuracy and Linearity.
Representative data for the IL-2Rβγ Bioassay. IL-2Rβγ Bioassay cells were prepared as described in the protocol and incubated with serial dilutions of recombinant IL-2 and IL-15. After a 6-hour incubation, Bio-Glo-NL™ Reagent was added, and luminescence was quantified using the GloMax® Discover System. Data were fitted to a 4PL curve using GraphPad Prism® software. The IL-2 EC 50 was 81ng/ml ( Panel A ), with a fold induction of approximately 24 ( Panel B ). The IL-15 EC 50 was 4.4ng/ml with a fold induction of approximately 28. Data were generated using cells from culture. Panel A shows raw luminescence measurements. Panel B displays the calculated fold induction.