Promega, JA6101, CD28 Blockade Bioassay

Assay Performance Data
Accuracy and precision are critical to bioassays and the development of biologics. The CD28 Blockade Bioassay is both accurate and precise. Accuracy is a measure of how close results are to a target or reference value. Precision is a measure of repeatability. This assay was developed and prequalified according to ICH guidelines for accuracy and precision giving it the performance characteristics required for many applications of a bioassay used for antibody drug discovery research and development.
Accuracy: Measure of how close results are to a reference or "target" value.
IntraLab Precision: Measure of repeatability from multiple assays of the same homogeneous sample under the same operating conditions over a short time (same analyst; same lab; same day).
Intermediate Precision: Measure of repeatability of multiple assays of the same homogeneous sample with different analysts or equipment or on different days.
Linearity: Ability of the assay to provide measured values that are proportional to the concentration of the sample.
The CD28 Blockade Bioassay Shows Precision, Accuracy and Linearity.
The CD28 Blockade Bioassay reflects the mechanism of action (MOA) and specificity of biologics designed to block the CD28/B7 interaction.
CD28 Effector Cells were incubated with aAPC/Raji Cells in the presence of serial titrations of blocking Abs as indicated. After a 5-hour induction, Bio-Glo™ Reagent was added and luminescence quantified using the GloMax® Discover Detection System. Data were generated using thaw-and-use cells.
CD28 Effector Cells, a titration of Control Ab, Anti-CD28 (Cat.# K1231), and aAPC/Raji Cells were added to a 96-well assay plate. After a 5-hour incubation at 37°C, Bio-Glo™ Reagent was added and luminescence measured using the GloMax® Discover System.