Promega, JA9011, TLR Reporter Bioassay

Name: TLR Reporter Bioassay
Brand: Promega
SKU: JA9011

Measure Potency and Stability of Molecules that Modulate TLR Signaling, 96- and 384-well plate formats, No primary cell culture required, Supplied in convenient thaw-and-use format; also available in cell propagation model (CPM) format, Configuration: Thaw-and-Use/1X each component

Catalog Number: JA9011

Pricing

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Category

Cell Detachment Solutions

TagsBioassays Macrophage-Directed Bioassays

Description

TLR Bioassay: Detect Pyrogens with Precision
What is a pyrogen detection assay?
A pyrogen detection assay is a test used to identify substances that can cause inflammatory responses when introduced into the human body. These substances, known as pyrogens, can originate from bacteria, fungi, or other sources and are recognized by Toll-like receptors (TLRs) and pose serious safety risks when found in pharmaceutical products. Pyrogen detection assays ensure product safety by verifying that therapeutics are free from contaminants before they reach clinical or commercial use.
What are traditional options for pyrogen testing?
Limulus Amebocyte Lysate (LAL): Uses horseshoe crab blood that is highly sensitive to endotoxin. Limited utility to non-endotoxins and presents sustainability concerns.
Uses horseshoe crab blood that is highly sensitive to endotoxin.
Limited utility to non-endotoxins and presents sustainability concerns.
Primary Cell-Based Cytokine Assays: Uses primary monocytes to measure cytokine in response to pyrogen exposure. Highly variable due to donor biology and lengthy protocols.
Uses primary monocytes to measure cytokine in response to pyrogen exposure.
Highly variable due to donor biology and lengthy protocols.
Rabbit Pyrogen Test (RPT): Uses live rabbits to detect an immune response post injection. Relies on live animals, limited accuracy, and poor reproducibility.
Uses live rabbits to detect an immune response post injection.
Relies on live animals, limited accuracy, and poor reproducibility.
How does the TLR Bioassay provide an improved pyrogen detection assay?
Physiologically Relevant: Uses an engineered THP-1 monocytic cell line without donor variability.
Comprehensive Detection: Identifies both endotoxin and non-endotoxin pyrogens that signals through TLR 1,2,4,5,6.
Ethical and Sustainable: Eliminates the need for animal-based testing methods.
Thaw-and-Use Format: Streamlined, cell-based assay with standardized reagents for rapid results.
Promega cell-based assays have been the market leader for years. See how the TLR Bioassay continues that tradition in new applications.

Specifications

{"type": "table", "rows": [{"name": "TLR Bioassay Cells", "conditions": {"Part #": "GA132A", "Size": "1 × 0.5ml"}}, {"name": "Fetal Bovine Serum", "conditions": {"Part #": "J121A", "Size": "1 × 4ml"}}, {"name": "RPMI 1640 Medium", "conditions": {"Part #": "G708A", "Size": "1 × 36ml"}}, {"name": "Bio-Glo-NL™ Luciferase Assay Buffer", "conditions": {"Part #": "J308A", "Size": "1 × 10ml"}}, {"name": "Bio-Glo-NL™ Luciferase Assay Substrate", "conditions": {"Part #": "J309A", "Size": "1 × 200μl"}}]}

Ordering

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