Description
Translation Optimization for Mg2+ and K+ Concentrations
The Flexi® Rabbit Reticulocyte Lysate System allows translation reactions to be optimized for a wide range of parameters, including Mg 2+ and K + concentrations and the choice to add or omit DTT. To help optimize Mg 2+ for a specific message, the endogenous Mg 2+ concentration of each lysate batch is stated in the product information included with this product. The Flexi® System also offers the choice of three amino acid mixtures and includes a control RNA encoding the firefly luciferase gene.
The Flexi® Rabbit Reticulocyte Lysate System, when optimized, can increase the quantity of protein produced as much as fourfold over a standard lysate reaction. The Flexi® System contains three Amino Acid Mixtures, enabling different choices of radioisotopes. Luciferase Control RNA and Luciferase Assay Reagent are included with the system as a functional control (only full-length luciferase is active).
For more information, see the Protocols & Applications Guide .
Applications:
Optimizing translation of low-expressing messages.
Oxidizing environment can be adjusted, which may be important for maintaining functional activity related to disulfide bridges.
Luciferase activity can be used for the detection of inhibitory contaminants in RNA preparations.
Investigation of translational control.
Screening of chemical libraries for effect on translation.
Reference:
Beckler, G.S. et al. (1995) Methods in Molecular Biology 37 , 215–32.
