Promega, M1804, T4 DNA Ligase

Name: T4 DNA Ligase
Brand: Promega
SKU: M1804

Joins Two DNA Strands with Cohesive or Blunt Ends, Use with 5´, 3´ or blunt-ended dsDNA (e.g., inserts and vectors), Qualified for blue/white cloning, providing a higher level of quality control for enzymes used in cloning applications, Can catalyze the joining of RNA to either a DNA or RNA strand in a duplex molecule, Intended for Laboratory Use (GPR) in the United States. Outside of the United States, this product is intended for research use only unless otherwise stated, Configuration: 1–3u/µ

Catalog Number: M1804

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Category

Modifying Enzymes

TagsCloning and DNA Markers Molecular Biology Enzymes and Reagents

Description

T4 DNA Ligase catalyzes the joining of two cohesive- or blunt-ended strands of DNA between the 5´-phosphate and the 3´-hydroxyl groups of adjacent nucleotides. The enzyme will not join single-stranded nucleic acids. T4 DNA Ligase is provided with 10X Reaction Buffer: 300mM Tris-HCl (pH 7.8 at 25°C), 100mM MgCl 2 , 100mM DTT and 10mM ATP.
Reference
Engler, M.J. and Richardson, C.C. (1982) In: The Enzymes , Boyer, P.D., ed., Academic Press, New York, NY.
Note: Units listed are Weiss units.
Storage Buffer: 10mM Tris-HCl (pH 7.4 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA and 50% glycerol.
Source: Recombinant E. coli strain.
QC Tests: Activity, SDS-PAGE/purity, DNase, endonuclease, RNase, blue/white cloning assay.
Unit Definition: 0.01 Weiss unit of T4 DNA Ligase is the amount of enzyme required to catalyze the ligation of greater than 95% of 1μg of λ/HindIII fragments at 16°C in 20 minutes.

Specifications

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