Promega, M3011, Single-Stranded DNA Binding Protein

Name: Single-Stranded DNA Binding Protein
Brand: Promega
SKU: M3011

Cooperatively Binds ssDNA with High Affinity, Consists of four identical 18.9kDa subunits, Does not bind well to double-stranded DNA, Supplied at a concentration of 1–5µg/μl, Configuration: 100μg

Catalog Number: M3011

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Category

Modifying Enzymes

TagsCloning and DNA Markers Molecular Biology Enzymes and Reagents

Description

E. coli Single-Stranded DNA Binding Protein (SSB) consists of four identical 18.9kDa subunits that cooperatively bind to single-stranded DNA with high affinity. This protein is involved in DNA replication and in recombination in vivo.
Applications
Studying DNA replication and recombination.
DNA sequencing using the primer walking strategy with contiguous oligonucleotides.
References
Sancar, A. et al. (1981) Proc. Natl. Acad. Sci. USA 78 , 4274–8.
Chase, J.W. and Williams, K.R. (1986) Ann. Rev. Biochem. 55 , 103–36.
Krauss, G. et al. (1981) Biochemistry 20 , 5346–52.
Weiner, J.H. et al. (1975) J. Biol. Chem. 250 , 1972–80.
Storage Buffer: 50mM Tris-HCl (pH 7.4), 1mM EDTA, 1mM DTT, 0.2M NaCl, 50% (v/v) glycerol.
Source: E. coli.
QC Tests: Protein concentration, DNase, RNase, SDS-PAGE/purity, endonuclease.

Specifications

General

Catalog Number	M3011

Ordering

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