Description
RNase ONE™ Ribonuclease is a 27kDa periplasmic enzyme from E. coli that catalyzes the degradation of RNA to cyclic nucleotide monophosphate (NMP) intermediates. Slower hydrolysis further catalyzes the degradation of these intermediates to 3´-NMPs. RNase ONE™ Ribonuclease may be used to remove RNA from DNA preparations, for mismatch detection, RNase protection assays and mapping, or quantitation of RNA by selective cleavage of single-stranded regions. The enzyme is provided with 10X Reaction Buffer: 100mM Tris-HCl (pH 7.5 at 25°C), 50mM EDTA, 2M sodium acetate.
Shen, V. and Schlessinger, D. (1982) The Enzymes , Vol. XV, 501.
Meador, J., 3rd et al. (1990) Eur. J. Biochem. 187 , 549–53.
Meador, J., 3rd and Kennell, D. (1990) Gene 95 , 1–7.
Storage Buffer: 10mM Tris-HCl (pH 8.0), 200mM NaCl, 50% (v/v) glycerol.
Source: Recombinant E. coli strain.
QC Tests: Activity, SDS-PAGE/purity, endonuclease, DNase.
Unit Definition: One unit is defined as the amount of enzyme required tocompletely degrade RNA at the rate of 100ng/second at 37°C in 10mM Tris-HCl (pH 7.5 at 25°C), 5mM EDTA, 200mM sodium acetate, 0.2μg/μl RNA, 0.05% NP-40 and 2μg/μl BSA.
