Promega, M4285, Ribonuclease H

Name: Ribonuclease H
Brand: Promega
SKU: M4285

Endonuclease that Specifically Hydrolyzes the Phosphodiester Bonds of RNA Hybridized to DNA, Removes the RNA strand prior to second-strand cDNA synthesis, Analyzes in vitro polyadenylation reaction products, Supplied at a concentration of 0.5–2u/μl, Intended for Laboratory Use (GPR) in the United States. Outside of the United States, this product is intended for research use only unless otherwise stated, Configuration: 250u

Catalog Number: M4285

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Category

Modifying Enzymes

TagsCloning and DNA Markers Molecular Biology Enzymes and Reagents

Description

Ribonuclease H (RNase H) is an endonuclease that specifically hydrolyzes the phosphodiester bonds of RNA hybridized to DNA to produce 3′-OH and 5′-P-terminated products. It will not degrade single-stranded nucleic acids, double-stranded DNA or double-stranded RNA. This endonuclease can be used in applications such as microarrays.
Reference
Goodwin, E.C. and Rottman, F.M. (1992) Nucl. Acids Res. 20 , 916.
Storage Buffer: 20mM HEPES-KOH (pH 7.8 at 25°C), 1mM DTT, 50mM KCl, 50% (v/v) glycerol, 0.2mg/ml BSA.
Source: Recombinant E. coli strain.
QC Tests: Activity, DNase, RNase, endonuclease, cDNA synthesis.
Unit Definition: One unit is defined as the amount of enzyme required to produce 1nmol of acid-soluble ribonucleotides from radiolabeled poly(rA):poly(dT) in 20 minutes at 37°C in 20mM HEPES-KOH (pH 7.8), 50mM KCl, 10mM MgCl 2 , 1mM DTT, 20μM radiolabeled poly(rA):poly(dT).

Specifications

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