Promega, M5313, M-MLV Reverse Transcriptase

Name: M-MLV Reverse Transcriptase
Brand: Promega
SKU: M5313

RNA-Dependent DNA Polymerase for cDNA Synthesis, Enzyme supplied at a concentration of 200u/μl, Configuration: Buffer Pack/2 × 1ml

Catalog Number: M5313

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Category

Reverse Transcriptases

TagsPCR RT-PCR

Description

RNA-Dependent DNA Polymerase for cDNA Synthesis
Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) is an RNA-dependent DNA polymerase that can be used in cDNA synthesis with long messenger RNA templates (>5kb). The enzyme is a product of the pol gene of M-MLV and consists of a single subunit with a molecular weight of 71kDa. The RNase H activity of M-MLV RT is weaker than that of the commonly used Avian Myeloblastosis Virus (AMV) reverse transcriptase.
Provided with 5X Reaction Buffer: 250mM Tris-HCl (pH 8.3 at 25°C), 375mM KCl, 15mM MgCl 2 , 50mM DTT.
May Be Heat-Inactivated: M-MLV RT is inactivated by heating at 70°C for 10 minutes.

Specifications

{"type": "table", "rows": [{"name": "M-MLV RT 5X Buffer", "conditions": {"Part #": "M531A", "Size": "2 × 1ml"}}]}

Ordering

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