Description
S1 Nuclease is an endonuclease that degrades ssDNA and RNA. The enzyme is used to remove protruding single-stranded termini from double-stranded DNA, for selective cleavage of single-stranded DNA and for mapping RNA transcripts. S1 Nuclease is provided with 10X Reaction Buffer: 0.5M sodium acetate (pH 4.5 at 25°C), 2.8M NaCl, 45mM ZnSO 4 .
References
Vogt, V.M. (1973) Eur. J. Biochem. 33 , 192–200.
Roberts, T.M. et al. (1979) Proc. Natl. Acad. Sci. USA 76 , 760–4.
Berk, A.J. and Sharp, P.A. (1978) Proc. Natl. Acad. Sci. USA 75 , 1274–8.
Storage Buffer: 20mM Tris-HCl (pH 7.5 at 25°C), 0.1mM ZnCl 2 , 50mM NaCl and 50% (v/v) glycerol.
Source: Fungal α amylase powder.
QC Tests: Activity, unidirectional deletions using the Erase-a-Base™ System.
Unit Definition: One unit is defined as the amount of enzyme required to produce 1μg of acid-soluble material per minute at 37°C in 30mM sodium acetate (pH 4.6 at 25°C), 50mM NaCl, 1mM ZnCl 2 , 0.5mg/ml denatured calf thymus DNA and 5% glycerol.
