Description
What Are GoTaq® Master Mixes?
GoTaq® Master Mixes are premixed, ready-to-use 2X PCR reagent solutions designed for fast, reliable endpoint PCR. Each master mix contains GoTaq® DNA Polymerase, dNTPs, MgCl₂, and reaction buffer at concentrations optimized for efficient amplification of DNA templates. You add only your template DNA, primers, and nuclease-free water to set up a reaction in under a minute.
GoTaq® DNA Polymerase is a proprietary formulation of Taq DNA polymerase that provides robust amplification equal to, and in some cases superior to, standard Taq. Because the master mix is formulated for broad compatibility, you can drop it into your existing Taq-based PCR protocols without changing cycling parameters.
What Is the Difference Between GoTaq® Green and GoTaq® Colorless Master Mix?
The two GoTaq® Master Mix formulations share the same core chemistry: identical polymerase, buffer, dNTPs, and MgCl₂ concentration. The difference is in the tracking dyes:
GoTaq® Green Master Mix contains two dyes (blue and yellow) that serve as gel-loading indicators. The blue dye co-migrates with 3–5kb DNA fragments in a 1% agarose gel; the yellow dye migrates ahead of primers (<50bp). Reactions have enough density to load directly onto agarose gels after amplification. You don't need a separate loading dye or spin-down step.
GoTaq® Colorless Master Mix omits the tracking dyes entirely. This makes it the right choice when downstream analysis involves fluorescence or absorbance measurements (e.g., quantification by spectrophotometry at A260), because the blue and yellow dyes absorb between 225–300nm and can interfere with optical readings.
GoTaq® Green vs. Colorless
How Do I Set Up a PCR Reaction with GoTaq® Master Mix?
Setting up a GoTaq® Master Mix reaction takes less than a minute. The master mix comes as a 2X concentrate, so you assemble the final reaction by combining equal volumes of master mix and sample components. A standard 25µl reaction uses 12.5µl of GoTaq® Master Mix.
