Promega, N1481, Coincidence Reporter Vectors

Name: Coincidence Reporter Vectors
Brand: Promega
SKU: N1481

Vectors That Express Both Firefly and NanoLuc® Luciferases from the Same Transcript, Firefly and NanoLuc® luciferase have dissimilar compound interference profiles for better identifying more false-positives versus individually, Using two different transcriptional reporters reduces false hit rates and increases the identification of true biological hits, luc2, and, NlucP, provide a bright reporter combination compatible with low-copy-number and plate scale-up, and provide greater signal-to-backg

Catalog Number: N1481

Pricing

Send an RFQ.

View RFQ List

Category

Engineered Cell Lines

TagsLuciferase Assays Genetic Reporter Vectors and Cell Lines

Description

pNLCoI reporter vectors express the firefly luciferase and the destabilized NanoLuc® luciferase reporters on the same mRNA transcript. The two reporters are separated by a short P2A peptide, which causes ribosome skipping and the generation of two soluble, unfused forms of luciferase with distinct inhibitor profiles.
Two Reporter Genes, One Transcript
Luciferase-based reporter-gene assays remain a useful and powerful method of high-throughput compound screening. However, false hits that result from direct interaction of compounds with the luciferase reporter can result in unnecessary follow-up efforts. The pNLCoI Vectors comprise a second-generation coincidence reporter vector system that allow expression of both firefly luciferase ( luc2 ) and NanoLuc® Luciferase fused to a PEST destabilization domain ( NlucP ) from the same mRNA transcript. The stoichiometric expression of both luciferases is achieved by use of the P2A sequence from porcine teschovirus-1, which promotes a ribosomal skip and expression of the two unfused enzymes with distinct compound interaction profiles. When used in high-throughput compound screening, false hits caused by direct interaction with one or the other luciferases can be distinguished from true hits that show a similar response for both, reducing workload associated with follow-up screens.
The pNLCoI Vectors are designed for use with the Nano-Glo® Dual-Luciferase® Reporter (NanoDLR™) Assay System, which sequentially detects firefly and NanoLuc® Luciferase in activity in the same sample with a convenient “add-read-add-read” homogeneous format. Both NanoDLR™ reagents provide stable glow-type luminescence signals with half-lives of approximately two hours for automated batch processing of samples and amenable to assays or high-throughput screens in 96-, 384- or 1,536-well plate formats. Potent inhibition of firefly luciferase coupled with the high-intensity luminescence of NanoLuc® luciferase maximizes sensitivity for detection of both reporters.
pNLCoI1[ luc2 -P2A- NlucP /Hygro] Vector GenBank® Accession Number KM359771 and vector sequence text file .
pNLCoI2[ luc2 -P2A- NlucP /minP/Hygro] Vector GenBank® Accession Number KM359772 and vector sequence text file .
pNLCoI3[ luc2 -P2A- NlucP /CMV/Hygro] Vector GenBank® Accession Number KM359773 and vector sequence text file .
pNLCol4[ luc2 -P2A- NlucP /PGK/Hygro] Vector GenBank® Accession Number KM376434 and vector sequence text file .

Specifications

{"type": "table", "rows": [{"name": "pNLCoI3[ luc2 -P2A- NlucP /CMV/Hygro] Vector", "conditions": {"Part #": "N148A", "Size": "1 × 20μg"}}]}

Ordering

Notice

Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:

[email protected]

Please send inquiries directly to us in the “To” field. Emails sent via BCC, mass mailing, or with unrelated recipients may be automatically filtered and will not be processed.