Description
The NanoBRET® Positive Control Vector encodes a NanoLuc®-HaloTag® fusion protein that tethers together the NanoLuc® donor and HaloTag® acceptor proteins to ensure efficient energy transfer. The NanoBRET® Positive Control Vector should be diluted in Transfection Carrier DNA to reduce cellular expression levels due to the high NanoBRET® signal produced. The control vector can be used when performing instrument setup or as a reference control when performing other NanoBRET® assays.
The NanoBRET® Positive Control contains the following key features:
NanoLuc®-HaloTag® fusion gene.
Kanamycin resistance gene for selection in bacteria.
A CMV promoter for high-level expression of NanoLuc®-HaloTag® fusion in mammalian cells.
The Transfection Carrier DNA contains the following key features:
Ampicillin resistance gene for selection in bacteria.
