Description
The NanoBRET® PPI Control Pair (p53, MDM2) is an optimized pair of vectors designed to measure the interaction of protein partners p53 and MDM2 using the NanoBRET® assay. The p53, MDM2 assay gives a strong NanoBRET® signal and can be used when performing instrument setup or included as a reference control pair when performing other NanoBRET® PPI assays.
The p53-HaloTag® Fusion Vector contains the following key features:
p53-HaloTag® fusion gene.
Kanamycin resistance gene for selection in bacteria.
A CMV promoter for high-level expression of the p53-HaloTag® fusion in mammalian cells.
The NanoLuc®-MDM2 Fusion Vector contains the following key features:
NanoLuc®-MDM2 fusion gene.
A CMV promoter for high-level expression of the NanoLuc®-MDM2 fusion in mammalian cells.
p53 HaloTag® Fusion Vector ( sequence text file ).
NanoLuc® MDM2 Fusion Vector ( sequence text file ).
