Promega, N1821, NanoBRET® PPI Starter Systems

Name: NanoBRET® PPI Starter Systems
Brand: Promega
SKU: N1821

BRET-Based Assays to Detect Protein:Protein Interactions in Live Cells, Study induction and inhibition of protein interactions using full-length proteins expressed at physiologically relevant levels, Starter Systems provide fusion vectors, positive controls and detection reagents for building PPI assays, Live-cell detection options for endpoint and kinetic analysis, Configuration: Flexi

Catalog Number: N1821

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Category

Insect Cell Expression Systems

TagsProtein Interactions Live-Cell Protein Interactions

Description

Sensitive, Scalable Assays to Study Protein Interactions in the Natural Cellular Context
NanoBRET®-based protein:protein interaction (PPI) assays use NanoLuc® Luciferase as a BRET energy donor and HaloTag® protein, labeled with the NanoBRET® 618 fluorophore, as the energy acceptor to measure the interaction of specific protein pairs.
The NanoBRET® PPI Starter Systems provide the vectors required to create NanoLuc® Luciferase and HaloTag® protein fusions to target proteins of interest, a Positive Control Pair (p53, MDM2) and the NanoBRET® Nano-Glo® Detection System, which contains the substrate used by NanoLuc® Luciferase to generate the donor signal and the HaloTag® NanoBRET® 618 Ligand for the fluorescent energy acceptor.
Live-cell reagents allow you to detect protein:protein interactions in real time using full-length proteins or fragments.
Reversible assay technology allows you to study both induction and inhibition of protein interactions.
Assays can be performed in 96- or 384-well formats with low variability and high reproducibility.

Specifications

{"type": "table", "rows": [{"name": "pFN21A HaloTag® CMV Flexi® Vector", "conditions": {"Part #": "G2821", "Size": "1 × 20μg", "Concentration": ""}}, {"name": "pFC14K HaloTag® CMV Flexi® Vector", "conditions": {"Part #": "G9661", "Size": "1 × 20μg", "Concentration": ""}}, {"name": "pFN31K Nluc CMV-neo Flexi® Vector", "conditions": {"Part #": "N1321", "Size": "1 × 20μg", "Concentration": "0.1μg/μl"}}, {"name": "pFC32K Nluc CMV-neo Flexi® Vector", "conditions": {"Part #": "N1341", "Size": "1 × 20μg", "Concentration": "0.1μg/μl"}}, {"name": "NanoBRET® PPI Control Pair (p53, MDM2)", "conditions": {"Part #": "N1641", "Size": "1 × 2 × 20μg", "Concentration": ""}}, {"name": "NanoBRET® Nano-Glo® Detection System", "conditions": {"Part #": "N1661", "Size": "1 × 200 assays", "Concentration": ""}}]}

Ordering

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