Description
Example NanoBRET® BRD/Histone Protein Interaction Assay Data
Example BRD4 Assay Data: Monitoring Inhibition of Protein Interactions
This example shows the dose-dependent inhibition of BRD4 protein interaction with Histone H3.3 by JQ1 as measured by the decrease in the NanoBRET®™ signal.
Example BRD9 Assay Data: High Assay Specificity
In this donor saturation assay, cells were transfected with a constant amount of BRD9 FL- or BRD9 BD-NanoLuc® Fusion donor DNA and increasing amounts of Histone H3.3-HaloTag® Fusion acceptor DNA to represent increasing acceptor-to-donor (A:D) ratios. The negative control was a set of samples containing HaloTag® protein alone. Both NanoBRET® BRD9/Histone H3.3 Interaction Assays generate hyperbolic curves, which indicate detection of a specific BRET interaction.
Example BRPF1 Assay Data: Assay Specificity
This donor saturation assay was performed by transfecting cells with a constant amount of BRPF1- or BRPF1-NanoLuc® Fusion donor DNA and increasing amounts of Histone H4-HaloTag® Fusion acceptor DNA to represent increasing amounts of acceptor-to-donor (A:D) ratios. The negative control was HaloTag® protein alone in place of the Histone H4-HaloTag® fusion. The NanoBRET® BRPF1/Histone H4 Interaction Assays generate hyperbolic curves, which indicate detection of a specific BRET interaction.
