Description
NanoBiT® System Vectors
The NanoBiT® PPI Starter Systems provide the vectors required to create LgBiT and SmBiT protein fusions, along with control vectors and the Nano-Glo® Live Cell Assay System, a single-addition, non-lytic detection reagent used for monitoring NanoBiT® luminescence in living cells. See below for details of the vectors provided with each system.
Using the NanoBiT® MCS Starter System, you can generate N- and C-terminal LgBiT and SmBiT fusions to proteins of interest using traditional cloning with a multiple cloning site (MCS). Expression is driven by HSV-TK promoter, providing constitutive, low-level expression in mammalian cells.
The NanoBiT® Flexi® Starter System, allows you to generate N- and C-terminal LgBiT and SmBiT fusions using the Flexi® Vector Cloning System, a directional cloning method based on the rare-cutting restriction enzymes, SgfI and PmeI, that provides a rapid, efficient and high-fidelity way to transfer protein-coding regions between Flexi® Vectors without the need to resequence. The Find My Gene™ service contains many ORF clones already in Flexi® format for simple creation of fusions.
The NanoBiT® PPI Control Pair (FKBP:FRB; Cat.# N2016) is available separately as an inducible positive control.
Alternative CMV-based Starter Systems compatible with stable cell line generation, additional control options, βarrestin recruitment vectors and optimized assays for hot targets such as KRAS and EGFR.
