Promega, N2120, DNA Bundles for NanoBRET® Target Engagement HDAC and BET BRD Assays

Name: DNA Bundles for NanoBRET® Target Engagement HDAC and BET BRD Assays
Brand: Promega
SKU: N2120

For use with the NanoBRET® Target Engagement HDAC and BET BRD Assays, DNA Bundle designed for profiling against HDAC or BET BRD proteins in NanoBRET® TE Assay, Transfection-ready plasmids, Part of the NanoBRET® TE Intracellular HDAC (N2170) and BET BRD (N2180) Complete Kits available for separate purchase, Configuration: HDAC DNA Bundle/1 each

Catalog Number: N2120

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Category

BCA Protein Assay Kits

TagsEpigenetics Research Kits and Reagents Cell-Based and Biochemical Assays

Description

NanoBRET® Technology for Target Engagement
The NanoBRET® Target Engagement (TE) Intracellular HDAC and BET BRD Assays measure compound binding at select HDAC and BET bromodomain target proteins within intact cells. These target engagement assays are based on the NanoBRET® System, an energy transfer technique designed to measure molecular proximity in living cells. The NanoBRET® TE HDAC and BET BRD Assays analyze the apparent affinity of test compounds by competitive displacement of a NanoBRET® tracer reversibly bound to a NanoLuc® HDAC or BET BRD fusion protein in cells.

Specifications

{"type": "table", "rows": [{"name": "HDAC1-NanoLuc® FL Fusion Vector", "conditions": {"Part #": "N220A", "Size": "1 × 20μg"}}, {"name": "HDAC2-NanoLuc® FL Fusion Vector", "conditions": {"Part #": "N221A", "Size": "1 × 20μg"}}, {"name": "HDAC3-NanoLuc® FL Fusion Vector", "conditions": {"Part #": "N222A", "Size": "1 × 20μg"}}, {"name": "NanoLuc®-HDAC6 FL Fusion Vector", "conditions": {"Part #": "N223A", "Size": "1 × 20μg"}}, {"name": "NanoLuc®-HDAC6 CD2 Fusion Vector", "conditions": {"Part #": "N224A", "Size": "1 × 20μg"}}, {"name": "HDAC10-NanoLuc® FL Fusion Vector", "conditions": {"Part #": "N225A", "Size": "1 × 20μg"}}]}

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