Description
RNasin® Ribonuclease Inhibitor
Inhibits RNase A, B and C as well as human placental RNase.
Does not inhibit other RNases and modifying enzymes like reverse transcriptases.
Results in higher yield in RNA extractions and better performance in RT-PCR, cDNA synthesis, microarrays and in vitro transcription/translation.
RNasin® Plus Ribonuclease Inhibitor
Possesses all the proven features of Recombinant RNasin® Ribonuclease Inhibitor.
Stable against oxidation and heat, leading to higher stability—up to 15 minutes at 70 °C.
Particularly suitable for long-term storage of samples.
RNase Contamination Happens, Even When You Are Careful
RNases are ubiquitous and can cause RNA degradation and compromise RNA integrity. RNase contamination can happen during RNA purification and during routine downstream handling. RNasin® Ribonuclease Inhibitors inhibit RNase A family and human placental RNases by noncovalently binding to RNases.
In this experiment, nine buffer and water stock solutions from an RNA-free zone of an academic lab were tested for RNases. The samples were in use for 1-14 months. Thirty-five microliters of each sample was collected, split and mixed with 5 µg of RNA and RNase ONE™ 10X buffer. RNasin® Ribonuclease Inhibitor (40u) was added to one of the two samples, and both were incubated overnight at 37 °C. Lane 1, marker; lane 2, RNA control; lanes 3–11, samples from the laboratory.
Of the nine samples tested, seven showed varying degrees of RNase contamination. For all samples tested, the RNA remained intact during the overnight incubation at 37°C when Recombinant RNasin® Ribonuclease Inhibitor was added. Details of this experiment can be found in this technical article .
