Promega, N2530, NanoBRET® TE Intracellular Kinase Assays

Name: NanoBRET® TE Intracellular Kinase Assays
Brand: Promega
SKU: N2530

Catalog Number: N2530

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Category

Phosphatases & Kinases

TagsCell Signaling Kinase Target Engagement

Description

Quantitative, Wide-Spectrum Kinase Profiling in Live Cells for Assessing the Effect of Cellular ATP on Target Engagement
Extracellular techniques to measuring binding of kinase inhibitors can fail to accurately predict intracellular target engagement. In this article, published in Cell Chemical Biology , we report the application of an energy transfer technique that enables the first equilibrium-based approach to quantitatively profile target occupancy and compound affinity in live cells.
Discovery of a Selective Inhibitor of Doublecortin-Like Kinase 1
Doublecortin-like kinase 1 (DCLK1) is an understudied kinase that is upregulated in a wide range of cancers, including pancreatic ductal adenocarcinoma (PDAC). This study uses NanoBRET® technology to understand the function of DCLK1 in PDAC cells.
Quantifying CDK Inhibitor Selectivity in Live Cells
Teams at the Structural Genomics Consortium and Promega used the NanoBRET® Target Engagement technology to uncover surprising patterns of selectivity for touted CDKIs and abandoned clinical leads. The results offer exciting opportunities for repurposing some inhibitors as selective chemical probes for lesser-studied CDK family members.
View more publications featuring NanoBRET® TE technology

Specifications

General

Catalog Number	N2530

Ordering

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