Description
Detection Reagent for use with NanoBRET® Protein Interaction Assays
The NanoBRET® assay is a bioluminescence resonance energy transfer (BRET)-based assay that uses NanoLuc® Luciferase as the BRET energy donor and HaloTag® protein labeled with the HaloTag® NanoBRET® 618 fluorescent Ligand as the energy acceptor to measure the interaction of two binding partners in live cells.
The NanoBRET® Nano-Glo® Detection System provides the NanoBRET® Nano-Glo® Substrate used by NanoLuc® Luciferase to generate the donor signal and the HaloTag® NanoBRET® 618 Ligand for the fluorescent energy acceptor. The HaloTag® NanoBRET® 618 Ligand is added directly to the cells during plating, and the NanoBRET® Nano-Glo® Substrate is added to the sample just prior to measuring donor and acceptor emission. The NanoBRET® Nano-Glo® Kinetic Detection System has the Vivazine® substrate for use by the NanoLuc® luciferase donor, giving an extended signal for live-cell kinetic assays.
For more details on using the NanoBRET® assay for protein:protein interaction studies visit: NanoBRET® Technology for Protein Interactions . The NanoBRET® Nano-Glo® Detection Systems are compatible with a diverse set of prebuilt or custom NanoBRET® PPI Assays.
Note: The total number of assays will vary based on final well volume. For standard well volumes: Cat.# N1661 and N2583 are sufficient for 200 assays in 96-well plates or 500 assays in 384-well plates. Cat.# N1662 and N2584 are sufficient for 1,000 assays in 96-well plates or 2,500 assays in 384-well plates. Cat.# N1663 and N2585 are sufficient for 10,000 assays in 96-well plates or 25,000 assays in 384-well plates.
