Promega, N2590, Nano-Glo® Endurazine™ and Vivazine® Live Cell Substrates

Name: Nano-Glo® Endurazine™ and Vivazine® Live Cell Substrates
Brand: Promega
SKU: N2590

Live Cell Detection of NanoLuc® or NanoBiT® Reporter Activity for Hours or Days, Increased signal stability for extended real-time kinetic analysis of reporter activity, Simplify time course studies by measuring response in the same sample over time, Sensitivity to measure protein at endogenous levels—no overexpression required, Configuration: Both Substrates (Trial Size)/0.2ml

Catalog Number: N2590

Pricing

Send an RFQ.

View RFQ List

Category

Insect Cell Expression Systems

TagsLuciferase Assays Reporter Assays

Description

Overview of Nano-Glo® Live Cell Detection
Live-cell, nonlytic assays using the Nano-Glo® Live Cell Assay System are limited to ≤2 hours because of signal decay. The Nano-Glo® Endurazine™ and Vivazine® Live Cell Substrates provide alternative live-cell-detection methods for NanoLuc® and NanoBiT® luciferases that enable nonlytic assays for periods lasting several hours to days. For both substrates, a slow rate of ester hydrolysis leads to the steady release of furimazine throughout the experiment, a process catalyzed by cellular esterases. Once formed, furimazine serves as a substrate for NanoLuc® and NanoBiT® luciferases.
The Vivazine® substrate typically shows increased brightness but also an increased rate of signal decay compared to the Endurazine™ substrate. The Endurazine™ substrate will provide the maximum signal stability but lower initial signal intensity compared to the other Nano-Glo® Live Cell Substrates. Evaluate both substrates to determine which combination of signal intensity and stability is most suitable for your experiment [e.g., Nano-Glo® Extended Live Cell Substrate Trial Pack (Cat.# N2590)].
Choose the Best Combination of Signal Intensity and Stability
A plasmid encoding NanoLuc® luciferase expressed from the PGK promoter was transiently transfected into HEK293 cells and luminescence signal intensity was measured as indicated.
Assay Reporter Activity for Days
A plasmid encoding NanoLuc® luciferase expressed from the HSV-TK promoter was transiently transfected into HEK293 cells and luminescent signal intensity measured for 72 hours.

Specifications

{"type": "table", "rows": [{"name": "Nano-Glo® Endurazine™ Substrate, 100X", "conditions": {"Part #": "N257A", "Size": "1 × 0.1ml"}}, {"name": "Nano-Glo® Vivazine® Substrate, 100X", "conditions": {"Part #": "N258A", "Size": "1 × 0.1ml"}}]}

Ordering

Notice

Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:

[email protected]

Please send inquiries directly to us in the “To” field. Emails sent via BCC, mass mailing, or with unrelated recipients may be automatically filtered and will not be processed.