Promega, N3020, Nano-Glo® In-Gel Detection System

Name: Nano-Glo® In-Gel Detection System
Brand: Promega
SKU: N3020

Directly Detect NanoLuc® Fusion Proteins in Polyacrylamide Gels, Simply incubate native or SDS denaturing gels with reagent and image, No transfer to membranes required for detection, Eliminates the need for blocking or antibodies, Configuration: 100ml

Catalog Number: N3020

Pricing

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Category

BCA Protein Assay Kits

TagsLuciferase Assays Reporter Assays

Description

Specific and Sensitive Luminescent Detection
Eliminate the need for immunoblotting to detect NanoLuc® fusion proteins separated by polyacrylamide gel electrophoresis. Directly image gels after incubation with the Nano-Glo® In-Gel Detection Reagent. For native PAGE, the gels can be incubated with detection reagent and imaged in less than 15 minutes. For denaturing SDS-PAGE, two washes with 25% isopropanol followed by two washes in water are needed to remove the SDS and allow the NanoLuc® luciferase to refold before detection.
The sensitivity of NanoLuc® luciferase means proteins do not need to be overexpressed to be visualized by the Nano-Glo® In-Gel Detection System.
Easily detect NanoLuc® protein fusions to:
Verify expression levels
Confirm correct molecular weight
Multiplex measurements of multiple luminescent species
Overview of the Nano-Glo® In-Gel Detection System.

Specifications

General

NACRES	NA.47
PubChem Substance ID	24897617
UNSPSC Code	12171500
MDL number	MFCD00078493
InChI key	IFSXZLJQEKGQAF-UHFFFAOYSA-M
SMILES string	[Na+].Nc1c(O)c(c(O)c2C(=O)c3ccccc3C(=O)c12)S([O-])(=O)=O
InChI	1S/C14H9NO7S.Na/c15-9-7-8(12(18)14(13(9)19)23(20,21)22)11(17)6-4-2-1-3-5(6)10(7)16;/h1-4,18-19H,15H2,(H,20,21,22);/q;+1/p-1
form	solution
shelf life	Expiry date on the label
IVD	for in vitro diagnostic use
application(s)	hematologyhistology
storage temp.	room temp
Quality Level	500

Ordering

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