Promega, N3040, Nano-Glo® HiBiT Lytic Detection System

Precise Protein Quantification
The broad linear dynamic range accurately quantifies tagged proteins regardless of expression level to measure changes in protein abundance. With a limit of detection of less than 10 -19 moles, the Nano-Glo® HiBiT Lytic Detection System can quantify even low abundance proteins at endogenous levels of expression.
Titrating purified HiBiT-tagged protein to demonstrate linear dynamic range
Study Proteins Under Endogenous Regulation
Using CRISPR-Cas9 gene editing, HiBiT-tagged proteins can be expressed under endogenous regulatory conditions, reducing overexpression artifacts and maintaining proper stoichiometry with other endogenous binding partners or regulatory machinery.
The small 11 aa HiBiT tag can be added to the desired protein in one of two ways:
Traditional recombinant methods with your promoter of choice
Insertion into endogenous loci using a simple, clone-free CRISPR-Cas9 genome editing protocol