Promega, N372X, RNasin® Plus Ribonuclease Inhibitor

RNasin® Plus is Stable at Elevated Temperatures
RNasin® Plus RNase Inhibitor is a recombinant mammalian RNase inhibitor capable of inhibiting eukaryotic RNases, such as RNase A and RNase B, similar to the human version of the protein. Certain cysteine residues present in human placental RNase inhibitor have been implicated in the oxidation sensitivity of that enzyme (1). RNasin® Plus RNase Inhibitor does not contain these cysteine residues, which makes it more resistant to oxidative damage. RNasin® Plus RNase Inhibitor forms a stable complex with RNases, inactivating the RNase. This complex is stable at temperatures up to 70°C for at least 15 minutes (Figure 1).
RNasin® Plus has been tested in RT-PCR and, like the human protein, is compatible with enzymes like AMV, M-MLV or ImProm-II™ Reverse Transcriptases as well as Taq and Tfl DNA Polymerases. RNasin® Plus has also been tested and found to be compatible with quantitative real-time RT-PCR in a TaqMan® Assay, and can even preserve templates from degradation in a biological extract as complex as a rat liver protein extract, demonstrating a broad spectrum of RNase inhibition (2).
Kim, B.M., et al . R.T. (1999) Variants of ribonuclease inhibitor that resist oxidation. Protein Sci. 8 , 430–4.
Andrews, C . et al. (2003) RNasin® Plus RNase Inhibitor: New Protein for High Temperature RNase Inhibition . Promega Corporation.