Promega, N5100, NanoBRET® 590 Dyes

Create NanoBRET® TE Tracers for Your Target of Interest
NanoBRET® 590 Dyes Used in Early-Stage Drug Discovery Workflow
DNA-encoded libraries (DELs) provide unmatched chemical diversity and starting points for novel drug modalities. An article in Cell Chemical Biology presents a workflow that exploits the bifunctional attributes of DEL ligands as a platform to generate BRET probes for live-cell target-engagement studies. The combination of DEL and BRET technology accelerates hit-to-lead studies in a live-cell setting.
For more information, see: Teske, K.A. et al. (2023) DELs enable the development of BRET probes for target engagement studies in cells. Cell Chem. Biol. 30 , 1–12.
The NanoBRET® Target Engagement (TE) Assay measures compound binding at select target proteins within intact cells. This target engagement assay is based on the NanoBRET® System, an energy transfer technique designed to measure molecular proximity in living cells. The NanoBRET® Target Engagement Assay analyzes the apparent affinity of test compounds by competitive displacement of a NanoBRET® tracer reversibly bound to a NanoLuc® fusion protein in a cell.
We have a number of TE assays already developed for a large number of protein targets from a variety of protein classes. If there is no existing NanoBRET® TE Assay for your target of interest, use the NanoBRET® 590 Dyes to develop novel tracers to enable NanoBRET® TE assays for new targets.