Promega, NE1100, ViaScript® mRNA Transfection Reagent

Advanced Expression Control for Optimized Assays
Evenly adjust expression across your entire cell population by proportionately changing the amount of reporter mRNA transfected. Titrating reporter expression with ViaScript® mRNA Transfection Reagent enables easy assay window optimization without compromising transfection efficiency.
Serial dilution of eGFP mRNA results in uniform decreases in signal intensity. AsPC-1 (left), Jurkat (middle) and HCT-116 (right) cells were transfected with either a 4-fold dilution series of eGFP mRNA (top) or DNA plasmid (bottom). Transfection efficiency and GFP signal intensity were measured by flow cytometry. The tighter peaks after mRNA transfection indicate the transfected cells express at similar levels, and the rightward shift in the peaks with dilution indicate proportionate changes in expression levels across the cell population. Neither of these features were observed with DNA plasmid transfection. The percent GFP positive cells for each condition is listed in the tables.