Promega, NE2020, Nano-Glo® HiBiT Dual-Luciferase® Reporter System

Name: Nano-Glo® HiBiT Dual-Luciferase® Reporter System
Brand: Promega
SKU: NE2020

Catalog Number: NE2020

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Category

Insect Cell Expression Systems

TagsProtein Detection Protein quantification

Description

Use the Fluc Signal to Distinguish Specific and Nonspecific Compound Effects on Protein Levels
Selective HiBiT-BRD4 degradation with two PROTACs compared to general cellular toxicity. HEK293 cells were transiently transfected with a construct expressing the HiBiT-BRD4-IRES-luc2 cassette from the weak TK promoter. Cells were treated with titrating concentrations of MZ1 ( Panel A ), dBET1 ( Panel B ) or staurosporine ( Panel C ) before the Fluc (blue) and HiBiT (red) signals were measured using the lytic HiBiT NanoDLR™ Assay. MZ1 and dBET1 selectively link BRD4 to two different E3 ligase complexes for targeted degradation, and staurosporine causes general toxicity, resulting in loss of both HiBiT-BRD4 and firefly luciferase (Fluc).

Specifications

General

Catalog Number	NE2020

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