Promega, P1450, Riboprobe® Combination Systems

Name: Riboprobe® Combination Systems
Brand: Promega
SKU: P1450

Produce High-Specific-Activity RNA Probes or Defined RNA Transcripts, Choose from combination systems with T3/T7 or SP6/T7 RNA polymerases, SP6, T7 and T3 RNA Polymerases are extremely promoter-specific, allowing production of virtually homogeneous RNA using plasmid DNA as a template, Allows synthesis of RNA corresponding to the coding or noncoding strand of cloned DNA from a single plasmid construct, Configuration: T3/T7

Catalog Number: P1450

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Category

DNA Polymerases

TagsRNA Analysis In vitro transcription

Description

The Riboprobe® Combination Systems are designed for in vitro preparation of high-specific-activity single-stranded RNA probes or microgram quantities of defined RNA transcripts from cloned DNA inserts. The Riboprobe® Combination Systems include the RNA polymerases, all of the required reagents (excluding radioisotope) for performing transcription reactions in vitro and RQ1 RNase-Free DNase (Cat.# M6101) for removal of the template following transcription.
Applications of the Riboprobe® Systems include:
Generation of high-specific-activity RNA probes.
Generation of microgram quantities of RNA transcripts.
Generation of RNA processing substrates.
Generation of antisense RNA.

Specifications

{"type": "table", "rows": [{"name": "RQ1 RNase-Free DNase", "conditions": {"Part #": "M610C", "Size": "1 × 110u", "Concentration": "", "Available Separately": "View Product"}}, {"name": "Recombinant RNasin® Ribonuclease Inhibitor", "conditions": {"Part #": "N251A", "Size": "1 × 2,500u", "Concentration": "40u/μl", "Available Separately": "View Product"}}, {"name": "rATP", "conditions": {"Part #": "P113A", "Size": "2 × 50μl", "Concentration": "10mM", "Available Separately": "View Product"}}, {"name": "rCTP", "conditions": {"Part #": "P114A", "Size": "2 × 50μl", "Concentration": "10mM", "Available Separately": "View Product"}}, {"name": "rGTP", "conditions": {"Part #": "P115A", "Size": "2 × 50μl", "Concentration": "10mM", "Available Separately": "View Product"}}, {"name": "rUTP", "conditions": {"Part #": "P116A", "Size": "2 × 50μl", "Concentration": "10mM", "Available Separately": "View Product"}}, {"name": "DTT", "conditions": {"Part #": "P117A", "Size": "2 × 100μl", "Concentration": "100mM", "Available Separately": "View Product"}}, {"name": "Transcription Optimized 5X Buffer", "conditions": {"Part #": "P118B", "Size": "1 × 500μl", "Concentration": "", "Available Separately": "View Product"}}, {"name": "Nuclease-Free Water", "conditions": {"Part #": "P119A", "Size": "2 × 1,250μl", "Concentration": "", "Available Separately": "View Product"}}, {"name": "T7 RNA Polymerase", "conditions": {"Part #": "P207D", "Size": "1 × 500u", "Concentration": "", "Available Separately": "View Product"}}, {"name": "T3 RNA Polymerase", "conditions": {"Part #": "P208A", "Size": "1 × 500u", "Concentration": "", "Available Separately": "View Product"}}, {"name": "pGEM® Express Positive Control Template", "conditions": {"Part #": "P256A", "Size": "1 × 5μg", "Concentration": "", "Available Separately": "View Product"}}]}

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