Promega, P1460, Riboprobe® Combination Systems

Name: Riboprobe® Combination Systems
Brand: Promega
SKU: P1460

Produce High-Specific-Activity RNA Probes or Defined RNA Transcripts, Choose from combination systems with T3/T7 or SP6/T7 RNA polymerases, SP6, T7 and T3 RNA Polymerases are extremely promoter-specific, allowing production of virtually homogeneous RNA using plasmid DNA as a template, Allows synthesis of RNA corresponding to the coding or noncoding strand of cloned DNA from a single plasmid construct, Configuration: SP6/T7

Catalog Number: P1460

Pricing

Send an RFQ.

View RFQ List

Category

DNA Polymerases

TagsRNA Analysis In vitro transcription

Description

The Riboprobe® Combination Systems are designed for in vitro preparation of high-specific-activity single-stranded RNA probes or microgram quantities of defined RNA transcripts from cloned DNA inserts. The Riboprobe® Combination Systems include the RNA polymerases, all of the required reagents (excluding radioisotope) for performing transcription reactions in vitro and RQ1 RNase-Free DNase (Cat.# M6101) for removal of the template following transcription.
Applications of the Riboprobe® Systems include:
Generation of high-specific-activity RNA probes.
Generation of microgram quantities of RNA transcripts.
Generation of RNA processing substrates.
Generation of antisense RNA.

Specifications

{"type": "table", "rows": [{"name": "RQ1 RNase-Free DNase", "conditions": {"Part #": "M610C", "Size": "1 × 110u", "Concentration": "", "Available Separately": "View Product"}}, {"name": "Recombinant RNasin® Ribonuclease Inhibitor", "conditions": {"Part #": "N251A", "Size": "1 × 2,500u", "Concentration": "40u/μl", "Available Separately": "View Product"}}, {"name": "rATP", "conditions": {"Part #": "P113A", "Size": "2 × 50μl", "Concentration": "10mM", "Available Separately": "View Product"}}, {"name": "rCTP", "conditions": {"Part #": "P114A", "Size": "2 × 50μl", "Concentration": "10mM", "Available Separately": "View Product"}}, {"name": "rGTP", "conditions": {"Part #": "P115A", "Size": "2 × 50μl", "Concentration": "10mM", "Available Separately": "View Product"}}, {"name": "rUTP", "conditions": {"Part #": "P116A", "Size": "2 × 50μl", "Concentration": "10mM", "Available Separately": "View Product"}}, {"name": "DTT", "conditions": {"Part #": "P117A", "Size": "2 × 100μl", "Concentration": "100mM", "Available Separately": "View Product"}}, {"name": "Transcription Optimized 5X Buffer", "conditions": {"Part #": "P118B", "Size": "1 × 500μl", "Concentration": "", "Available Separately": "View Product"}}, {"name": "Nuclease-Free Water", "conditions": {"Part #": "P119A", "Size": "2 × 1,250μl", "Concentration": "", "Available Separately": "View Product"}}, {"name": "SP6 RNA Polymerase", "conditions": {"Part #": "P108E", "Size": "1 × 500u", "Concentration": "", "Available Separately": "View Product"}}, {"name": "T7 RNA Polymerase", "conditions": {"Part #": "P207D", "Size": "1 × 500u", "Concentration": "", "Available Separately": "View Product"}}, {"name": "pGEM® Express Positive Control Template", "conditions": {"Part #": "P256A", "Size": "1 × 5μg", "Concentration": "", "Available Separately": "View Product"}}]}

Ordering

Notice

Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:

[email protected]

Please send inquiries directly to us in the “To” field. Emails sent via BCC, mass mailing, or with unrelated recipients may be automatically filtered and will not be processed.