Promega, P2083, T3 RNA Polymerase

Name: T3 RNA Polymerase
Brand: Promega
SKU: P2083

DNA-Dependent RNA Polymerase Specific for T3 Promoter Sequences, Exhibits extremely high affinity and specificity for T3 promoter sequences, >90% pure as determined by SDS polyacrylamide gel electrophoresis, Free of detectable levels of contaminating RNase and DNase activity (<1% release), Supplied at a concentration of 10–20u/μl (Cat.# P2083) and 80u/µl (Cat.# P4024), Configuration: 1, 000u

Catalog Number: P2083

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Category

Modifying Enzymes

TagsCloning and DNA Markers Molecular Biology Enzymes and Reagents

Description

T3 RNA Polymerase is a DNA-dependent RNA polymerase that synthesizes RNA using only T3 DNA or DNA templates cloned downstream from a T3 promoter. The polymerase can incorporate 32 P, 33 P, 3 H and 35 S nucleoside triphosphates during transcription. T3 RNA Polymerase is provided with 100mM DTT and Transcription Optimized 5X Buffer: 200mM Tris-HCl (pH 7.9 at 25°C), 30mM MgCl 2 , 10mM spermidine, 50mM NaCl.
Applications
Generation of templates for in vitro translation.
Generation of probes for nucleic acid hybridizations.
Generation of RNA processing substrates.
Generation of antisense RNA.
References
Butler, E.T. and Chamberlin, M.J. (1982) J. Biol. Chem. 257 , 5772–8.
Melton, D.A. et al. (1984) Nucl. Acids Res. 12 , 7035–56.
Storage Buffer: 20mM potassium phosphate (pH 7.7 at 25°C), 1.0mM EDTA, 10mM DTT, 100mM NaCl, 0.1% (v/v) Triton® X-100 and 50% (v/v) glycerol.
Source: Recombinant E. coli strain.
QC Tests: Activity, SDS-PAGE/purity, DNase, RNase, endonuclease, transcription.
Unit Definition: One unit is defined as the amount of enzyme required to catalyze the incorporation of 5nmol of CTP into acid-insoluble product in 60 minutes at 37°C in a total volume of 100μl. The reaction conditions are: 40mM Tris-HCl (pH 7.9 at 25°C), 6mM MgCl 2 , 10mM DTT, 10mM NaCl, 2mM spermidine, 0.05% Tween® 20, 0.5mM each of ATP, GTP, CTP, and UTP, 0.5μCi [ 3 H]CTP and 2μg supercoiled pSP6/T3 vector DNA.

Specifications

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